NMR Primer: An HSQC-based Approach with Vector AnimationsIM Publications, 2008 - 129 pages "This book has one aim: to explain the key two-dimensional protein NMR experiment, the 1H,15N-HSQC, along with variants and extensions, in a generally accessible manner. Vector diagrams of one-, two- and three-dimensional pulse sequences are provided, along with accompanying animated versions. The animations allow the evolution of net magnetisation during the course of the experiments to be visualised and directly compared with the corresponding spin operator terms. First, a brief introduction to spins, populations, the NMR experiment and relaxation is provided. Evolution due to J-coupling is next described and used to explain magnetisation transfer in the HSQC experiment and several variants. The extraction of structural, sequential and dynamic information is then illustrated via various extensions of the HSQC. Extensive footnotes and appendices introduce several more advanced concepts, such as sensitivity enhancement and the TROSY effect."--Publisher's description. |
Table des matières
The simplest onedimensional nMr experiment | 1 |
Two additional simple nMr experiments measuring T1 and T2 | 11 |
Jcoupling and the InepT sequence element | 17 |
summary | 28 |
summary | 39 |
3d HSQCbased double resonance experiments | 41 |
3d HSQCbased triple resonance experiments | 51 |
2d HSQCbased 15n relaxation experiments | 65 |
StatesTPPI | 79 |
More complex motions and relaxation | 88 |
summary | 100 |
Relaxationoptimised INEPT delays | 107 |
partial pulse sequence and concept bibliography | 113 |
Symbols and definitions | 119 |
125 | |
Fourier transform sign discrimination artefact suppression | 73 |
Expressions et termes fréquents
15N chemical shift 15N dimension 15N magnetisation 15N nucleus 15N pulse 15N spin 1H chemical 1H pulse 1JHN acquisition align amide protons amino acid artefacts B1 field Bloch-Siegert Chapter chemical shift evolution correlation decay decoupling dephasing deuterated dipolar coupling effect equilibrium evolution due Figure 4.2b flip frequency gradient GSE-HSQC gyromagnetic ratio Heteronuclear HN(CO)CA HNCA HNCO HSQC peak HSQC2 HxNz HyNz increment indirect dimension inverted J-coupling Journal of Magnetic magnetic field Magnetic Resonance molecule multiple quantum netisation NMR spectroscopy NOESY Nuclear Overhauser Effect nuclei NyHz phase cycling precession rate produces protein protein NMR pulse sequences Pulsed field gradients purge refocused relaxation result reverse INEPT rotating frame sample Schematic sensitivity enhancement shift is encoded simultaneous 90 spectral spectrometer spectroscopy spectrum t₁ T1 and T2 t1 period T₂ term tion TOCSY TPPI transfer magnetisation transverse TROSY TROSY-HSQC x-axis xy-plane z-axis zero