NMR Primer: An HSQC-based Approach with Vector Animations
IM Publications, 2008 - 129 pages
"This book has one aim: to explain the key two-dimensional protein NMR experiment, the 1H,15N-HSQC, along with variants and extensions, in a generally accessible manner. Vector diagrams of one-, two- and three-dimensional pulse sequences are provided, along with accompanying animated versions. The animations allow the evolution of net magnetisation during the course of the experiments to be visualised and directly compared with the corresponding spin operator terms. First, a brief introduction to spins, populations, the NMR experiment and relaxation is provided. Evolution due to J-coupling is next described and used to explain magnetisation transfer in the HSQC experiment and several variants. The extraction of structural, sequential and dynamic information is then illustrated via various extensions of the HSQC. Extensive footnotes and appendices introduce several more advanced concepts, such as sensitivity enhancement and the TROSY effect."--Publisher's description.
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3d HSQCbased double resonance experiments
Fourier transform sign discrimination artefact suppression
Transverse RelaxationOptimised SpectroscopY
BlochSiegert effect and BlochSiegert compensating pulses BSCP
Creating the cover image
15N chemical shift 15N dimension 15N magnetisation 15N nucleus 15N pulse 15N spin 1H chemical 1H pulse 1JHN 1JNC 2TNC align amide protons amino acid artefacts B1 field Bloch–Siegert Chapter correlation decay decoupling dephasing deuterated dipolar coupling discussed effect equation equilibrium evolution due Figure 4.2b flip GSE-HSQC gyromagnetic ratio Heteronuclear HN(CO)CA HNCA HNCO HSQC peak HxNz HyNz increment indirect dimension internal motion inverted J-coupling Journal of Magnetic magnetic field Magnetic Resonance measured molecule multiple quantum netisation NMR spectroscopy NOESY Nuclear Overhauser Effect nuclei NyCz NyHz pathway selection phase cycling precession rate produce protein protein NMR pulse sequences Pulsed field gradients purge quadrature detection radiation damping refocused relaxation result reverse INEPT rotating frame sample Schematic sensitivity enhancement simultaneous 90 spectral spectrometer spectroscopy spectrum T1 and T2 t1 period term tion TPPI transfer magnetisation transverse TROSY TROSY-HSQC tumbling vector x-axis xy-plane z-axis