Transgenic Crops of the World: Essential ProtocolsIan S. Curtis Springer Science & Business Media, 30 nov. 2004 - 454 pages Since the first transgenic plants were produced back in the early 1980s, there have been substantial developments towards the genetic engineering of most crops of our world. Initial studies using isolated plant cells and removing their cell walls to form protoplasts, offered the possibility of transferring genetic material by Agrobacterium-mediated gene transfer, chemical agents or electrical charges. However, in those cases were isolated protoplasts could be transformed, often, a shoot regeneration system was not available to induce the production of transgenic plants and any such regenerated plants were subject to mutation or chromosomal of cultured plant organs, such as leaf abnormalities. By the mid-1980s, the use disks, offered the convenience of combining gene transfer, plant regeneration and selection of transformants in a single system. This approach, enabled the production of stable, phenotypically-normal, transgenic potato and tomato plants in culture. By the late 1980s, the use of biolistics offered a means of inserting foreign genes into plant cells which where inaccessible to Agrobacterium infection. Even today, this technology is now standard practice for the production of some transgenic plants. |
Table des matières
Transgenic rice plants | 3 |
Transformation of wheat by biolistics | 19 |
Genetic transformation of barley Hordeum Vulgare L by coculture of immature embryos with Agrobacterium | 35 |
Maize transformation | 45 |
Genetic Engineering of Oat Avena Sativa L Via the Biolistic Bombarments of Shoot Apical Meristems | 63 |
Generation of Transgenic Rye | 79 |
Particle inflow gunmediated Transformation of Sorghum Biocolor | 89 |
Sugarcane Transformation | 103 |
Agrobacteriummediated genetic transformation of cotton | 243 |
Agrobacteriummediated transformation of potato | 257 |
Genetic transformation of radish Raphanus sativus L by floraldipping | 271 |
Genetic transformation of Allium cepa mediated by Agrobacterium Tumefaciens | 281 |
Transformation of carrot | 291 |
Production of transgenic cassava Manihot esculenta Crantz | 301 |
Soybean transformation using the Agrobacteriummediated cotyledonarynode method | 323 |
In vitro regeneration and transformation of Vicia Faba | 337 |
Biolistic Transformation of Fescues and Ryegrasses | 115 |
Transformation of Banana Using Microprojectile Bombardment | 131 |
AgrobacteriumMediated Transformation of Citrus | 145 |
Coffea SPP Genetic Transformation | 159 |
Genetic Transformation of Tea | 171 |
MicroprojectileMediated Transformation of Pineapple | 187 |
Regeneration and Genetic Transformation of Apple Malus Spp | 199 |
Genetic Transformation of Pear Via Agrobacteriummediated gene transfer | 217 |
AgrobacteriumMediated Transformation of Grape Embryogenic Calli | 229 |
Gene technology in pea | 351 |
Agrobacteriummediated transformation of cabbage | 361 |
Agrobacteriummediated transformation of canola | 379 |
Transformation of cauliflower | 389 |
Tomato transformationthe nuclear and chloroplast genomes | 405 |
Genetic transformation of watermelon | 425 |
Genetic transformation of sunflower | 435 |
Abbreviations | 453 |
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Expressions et termes fréquents
16 h photoperiod agar Agrobacterium tumefaciens Agrobacterium-mediated transformation antibiotics approx autoclaving bacterial biolistic Biotechnology bombardment Brassica breeding cabbage calli callus carbenicillin cassava cefotaxime co-cultivation cotyledons crop cultivars Culture Media distilled water Duchefa embryogenesis embryogenic embryogenic callus embryos ethanol explants expression faba filter-sterilized g/l sucrose gene transfer genetic engineering genetic transformation genome genotypes germination glasshouse growth gusA herbicide hygromycin hypocotyl immature embryos improvement Incubate kanamycin leaf maize meristems method mg/l kanamycin mg/ml stock microprojectile Molecular Note nptII organogenesis particles pear Petri dishes Plant Cell Plant Cell Reports plant regeneration plantlets plasmid potato protein protocol protoplasts regeneration medium resistance rice rifampicin root rootstocks seedlings seeds selectable marker selection medium Sigma somatic embryogenesis somatic embryos sorghum soybean species sterile stock solution store at 20°C sucrose sugarcane T-DNA tissue culture transformation efficiency transgenic plants tube tumefaciens strain vinifera vitamins vitro weeks